Environmental Exposures, Sperm Epigenetics and Early-life Development
Paternal Preconception Phthalate Exposure Alters Sperm Methylome and Embryonic Programming. Oluwayiose OA, Marcho C, Wu H, Houle E, Krawetz SA, Suvorov A, Mager J, Pilsner JR. Environment International, vol. 155, Oct. 2021, p. 106693., https://doi.org/10.1016/j.envint.2021.106693. PubMed **
**NIEHS Paper of the Month for September 2021
Peripubertal Serum Dioxin Concentrations and Subsequent Sperm Methylome Profiles of Young Russian Adults. Pilsner JR, Shershebnev A, Medvedeva YA, Suvorov A, Wu H, Goltsov A, Loukianov E, Andreeva T, Gusev F, Manakhov A, Smigulina L, Logacheva M, Shtratnikova V, Kuznetsova I, Speranskiy-Podobed P, Burns JS, Williams PL, Korrick S, Lee MM, Rogaev E, Hauser R, Sergeyev O. Reproductive Toxicology, vol. 78, June 2018, pp. 40–49., https://doi.org/10.1016/j.reprotox.2018.03.007. PubMed
Perinatal Exposure to Low Dose 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47) Alters Sperm DNA Methylation in Adult Rats. Suvorov A, Shershebnev A, Wu H, Medvedeva Y, Sergeyev O, Pilsner JR. Reproductive Toxicology, vol. 75, Jan. 2018, pp. 136–143., https://doi.org/10.1016/j.reprotox.2017.10.009. PubMed
Preconception Urinary Phthalate Concentrations and Sperm DNA Methylation Profiles among Men Undergoing IVF Treatment: A Cross-Sectional Study. Wu H, Estill MS, Shershebnev A, Suvorov A, Krawetz SA, Whitcomb BW, Dinnie H, Rahil T, Sites CK, Pilsner JR. Human Reproduction, vol. 32, no. 11, 12 Nov. 2017, pp. 2159–2169., https://doi.org/10.1093/humrep/dex283. PubMed
Paternal Preconception Phthalate Exposure Alters Sperm Methylome and Embryonic Programming. Wu H, Ashcraft L, Whitcomb BW, Rahil T, Tougias E, Sites CK, Pilsner JR. Environment International, vol. 155, Oct. 2021, p. 106693., https://doi.org/10.1016/j.envint.2021.106693. PubMed
Sperm Epigenetic Aging
Associations between Sperm Epigenetic Age and Semen Parameters: An Evaluation of Clinical and Non-Clinical Cohorts. Sawant S, Oluwayiose OA, Nowak K, Maxwell DL, Houle E, Paskavitz AL, Saddiki H, Bertolla RP, Pilsner JR. Curr Issues Mol Biol. 2024 Feb 16;46(2):1567-1578. doi: 10.3390/cimb46020101. PMID: 38392219; PMCID: PMC10887546. PubMed
Urinary phthalate metabolites and their mixtures are associated with advanced sperm epigenetic aging in a general population. Oluwayiose OA, Houle E, Wu H, Whitcomb BW, Mumford SL, Schisterman EF, Suvorov A, Balzer LB, Pilsner JR. Environ Res. 2022 Nov;214(Pt 4):114115. doi: 10.1016/j.envres.2022.114115. Epub 2022 Aug 19. PMID: 35988832. PubMed
Sperm epigenetic clock associates with pregnancy outcomes in the general population. Pilsner JR, Saddiki H, Whitcomb BW, Suvorov A, Buck Louis GM, Mumford SL, Schisterman EF, Oluwayiose OA, Balzer LB. Hum Reprod. 2022 Jun 30;37(7):1581-1593. doi: 10.1093/humrep/deac084. PMID: 35552703; PMCID: PMC9247414. PubMed
Small Non-Coding RNAs, Extracellular Vesicles, and Reproductive Health
Non-coding RNAs from seminal plasma extracellular vesicles and success of live birth among couples undergoing fertility treatment. Oluwayiose OA, Houle E, Whitcomb BW, Suvorov A, Rahil T, Sites CK, Krawetz SA, Visconti PE, Pilsner JR. Front Cell Dev Biol. 2023 Jun 22;11:1174211. doi: 10.3389/fcell.2023.1174211. PMID: 37427387; PMCID: PMC10323426. PubMed
Urinary phthalate metabolites and small non-coding RNAs from seminal plasma extracellular vesicles among men undergoing infertility treatment. Oluwayiose OA, Houle E, Whitcomb BW, Suvorov A, Rahil T, Sites CK, Krawetz SA, Visconti PE, Pilsner JR. Environ Pollut. 2023 Mar 30;329:121529. doi: 10.1016/j.envpol.2023.121529. Epub ahead of print. PMID: 37003585. PubMed
Altered non-coding RNA profiles of seminal plasma extracellular vesicles of men with poor semen quality undergoing in vitro fertilization treatment. Oluwayiose OA, Houle E, Whitcomb BW, Suvorov A, Rahil T, Sites CK, Krawetz SA, Visconti P, Pilsner JR. Andrology. 2023 May;11(4):677-686. doi: 10.1111/andr.13295. Epub 2022 Oct 2. PMID: 36111950; PMCID: PMC10017372. PubMed
Male Aging, Sperm Epigenetics,
and Reproductive Success
Age-associated epigenetic changes in mammalian sperm: implications for offspring health and development. Ashapkin V, Suvorov A, Pilsner JR, Krawetz SA, Sergeyev O. Hum Reprod Update. 2022 Sep 6:dmac033. doi: 10.1093/humupd/dmac033. Epub ahead of print. PMID: 36066418. PubMed
Urinary phthalate metabolites and their mixtures are associated with advanced sperm epigenetic aging in a general population. Oluwayiose OA, Houle E, Wu H, Whitcomb BW, Mumford SL, Schisterman EF, Suvorov A, Balzer LB, Pilsner JR. Environ Res. 2022 Nov;214(Pt 4):114115. doi: 10.1016/j.envres.2022.114115. Epub 2022 Aug 19. PMID: 35988832. PubMed
Aging-Induced Changes in Sperm DNA Methylation Are Modified by Low Dose of Perinatal Flame Retardants. Pilsner JR, Shershebnev A, Wu H, Marcho C, Dribnokhodova O, Shtratnikova V, Sergeyev O, Suvorov A. Epigenomics, vol. 13, no. 4, Feb. 2021, pp. 285–297., https://doi.org/10.2217/epi-2020-0404. PubMed
Sperm DNA Methylation Mediates the Association of Male Age on Reproductive Outcomes among Couples Undergoing Infertility Treatment. Oluwayiose OA, Wu H, Saddiki H, Whitcomb BW, Balzer LB, Brandon N, Suvorov A, Tayyab R, Sites CK, Hill L, Marcho C, Pilsner JR. Scientific Reports, vol. 11, no. 1, 5 Feb. 2021, https://doi.org/10.1038/s41598-020-80857-2. PubMed
Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant. Suvorov A, Pilsner JR, Naumov V, Shtratnikova V, Zheludkevich A, Gerasimov E, Logacheva M, Sergeyev O. International Journal of Molecular Sciences, vol. 21, no. 21, 4 Nov. 2020, p. 8252., https://doi.org/10.3390/ijms21218252. PubMed
Male Reproductive Biomarkers
Exploring the internal exposome of seminal plasma with semen quality and live birth: A Pilot Study. Houle E, Li Y, Schroder M, McRitchie SL, Rahil T, Sites CK, Jenkins Sumner S, Pilsner JR. Syst Biol Reprod Med. 2023 Apr 25:1-14. doi: 10.1080/19396368.2023.2195964. Epub ahead of print. PMID: 37098216. PubMed
Association between Sperm Mitochondarial DNA Copy Number and Nuclear DNA Methylation. Oluwayiose OA, Josyula S, Houle E, Marcho C, Brian W Whitcomb, Rahil T, Sites CK, Pilsner JR. Epigenomics, 15 Dec. 2020, https://doi.org/10.2217/epi-2020-0253. PubMed
Sperm Mitochondrial DNA Biomarkers and Couple Fecundity. Rosati AJ, Whitcomb BW, Brandon N, Buck Louis GM, Mumford SL, Schisterman EF, Pilsner JR. Human Reproduction, vol. 35, no. 11, 1 Nov. 2020, pp. 2619–2625., https://doi.org/10.1093/humrep/deaa191. PubMed
Sperm Mitochondrial DNA Measures and Semen Parameters among Men Undergoing Fertility Treatment. Wu H, Huffman AM, Whitcomb BW, Josyula S, Labrie S, Tougias E, Rahil T, Sites CK, Pilsner JR. Reproductive BioMedicine Online, vol. 38, no. 1, Jan. 2019, pp. 66–75., https://doi.org/10.1016/j.rbmo.2018.10.004. PubMed
Associations of Sperm Mitochondrial DNA Copy Number and Deletion Rate with Fertilization and Embryo Development in a Clinical Setting. Wu H, Whitcomb BW, Huffman A, Brandon N, Labrie S, Tougias E, Lynch K, Rahil T, Sites CK, Pilsner JR. Human Reproduction, vol. 34, no. 1, 14 Jan. 2018, pp. 163–170., https://doi.org/10.1093/humrep/dey330. PubMed
Reviews
Extracellular Vesicle Cargo of the Male Reproductive Tract and the Paternal Preconception Environment. Ayaz A, Houle E, Pilsner JR. Systems Biology in Reproductive Medicine, vol. 67, no. 2, 25 Apr. 2021, pp. 103–111., https://doi.org/10.1080/19396368.2020.1867665. PubMed
The Preconception Environment and Sperm Epigenetics. Marcho C, Oluwayiose OA, Pilsner JR. Andrology, vol. 8, no. 4, 21 Feb. 2020, pp. 924–942., https://doi.org/10.1111/andr.12753. PubMed
Environmental Susceptibility of the Sperm Epigenome during Windows of Male Germ Cell Development. Wu H, Hauser R, Krawetz SA, Pilsner JR. Current Environmental Health Reports, vol. 2, no. 4, 11 Dec. 2015, pp. 356–366., https://doi.org/10.1007/s40572-015-0067-7. PubMed
Method Development
Aclust2.0: a revamped unsupervised R tool for Infinium methylation beadchips data analyses. Oluwayiose OA, Wu H, Gao F, Baccarelli AA, Sofer T, Pilsner JR. Bioinformatics. 2022 Aug 26:btac583. doi: 10.1093/bioinformatics/btac583. Epub ahead of print. PMID: 36028931. PubMed
Optimization of Small RNA Extraction and Comparative Study of NGS Library Preparation from Low Count Sperm Samples. Shtratnikova V, Naumov V, Bezuglov V, Zheludkevich A, Smigulina L, Dikov Y, Denisova T, Suvorov A, Pilsner JR, Hauser R, Krawetz SA, Sergeyev O. Systems Biology in Reproductive Medicine, vol. 67, no. 3, 4 June 2021, pp. 230–243., https://doi.org/10.1080/19396368.2021.1912851. PubMed
Rapid Method for the Isolation of Mammalian Sperm DNA. Wu H, de Gannes MK, Luchetti G, Pilsner JR. BioTechniques, vol. 58, no. 6, June 2015, pp. 293–300., https://doi.org/10.2144/000114280. PubMed
Additional Publications
A complete list of publications can be found on PubMed.